Cloning and/or sequencing vector

ABSTRACT

A cloning and/or sequencing vector enables recombinant clones to be selected directly. The vector encodes a fusion protein which includes a protein poison.

RELATED APPLICATIONS

This application is a continuation application which claims priorityunder 35 U.S.C. § 120 to U.S. patent application Ser. No. 09/634,039,entitled CLONING AND/OR SEQUENCING VECTOR, filed Aug. 8, 2000, which isa continuation of U.S. patent application Ser. No. 09/225,152, entitledCLONING AND/OR SEQUENCING VECTOR, filed Jan. 4, 1999 and issued as U.S.Pat. No. 6,180,407, which is a continuation-in-part of U.S. patentapplication Ser. No. 08/379,614, entitled CLONING AND/OR SEQUENCINGVECTOR, filed Jul. 20, 1995 and issued as U.S. Pat. No. 5,910,438, whichis the U.S. National Phase under 35 U.S.C. § 371 of InternationalApplication PCT/BE93/00051, entitled CLONING AND/OR SEQUENCING VECTOR,filed Aug. 2, 1993 and published in English as PCT Publication No.WO94/03616, which claims priority to Belgian Application No. BE 9200696,filed Jul. 31, 1992.

SEQUENCE LISTING

The present application is being filed along with a Sequence Listing inelectronic format. The Sequence Listing is provided as a file entitledVANMA10-ICP1C3-SEQ.TXT, created Aug. 20, 2007, which is 10 Kb in size.The information in the electronic format of the Sequence Listing isincorporated herein by reference in its entirety.

SUBJECT-MATTER OF THE INVENTION

The invention relates to a cloning and/or sequencing vector whichenables recombinant clones to be selected directly.

The invention also relates to the procaryote cell which is transformedby this vector and to the procaryote host cell for this vector, as wellas to the use of this cloning and sequencing vector for selecting andsequencing recombinant clones.

STATE OF THE ART AND TECHNOLOGICAL BACKGROUND UNDERLYING THE INVENTION

Phage (the M13 series) and plasmid (the pUC series) cloning vectors,containing numerous unique cloning sites, were constructed by Messing etal (P.N.A.S. USA, 79, pp. 3642-3646 (1977), by Norrander et al (Gene,26, pp. 101-106 (1983) and Yanisch-Perron et al (Gene, 33 pp. 103 to119) (1985)).

The multiple cloning sites (MCS—multiple cloning sites) of these vectorsare located in the coding sequence of the LacZ gene.

Discrimination between the transformed cells which harbour a recombinantvector and the cells which harbour a non-recombinant vector is achievedusing the “blue screen” technique described by Gronenborn and Messing(Methylation of single-stranded DNA in vitro introduces new restrictionendonuclease cleavage sites, Nature, 272, pp. 375-377 (1978)).

However, this “blue screen” technique suffers from the disadvantage ofusing a screening procedure (discrimination) rather than a procedure forselecting the clones.

Discrimination by screening is based on identifying a clone within apopulation of clones on the basis of a characteristic (color) whichdifferentiates it. Selection has no need of this characteristic, sinceit is only recombinant clones which are isolated by this method.

The screening procedure is based on the color of the recombinant clones(white color) and of the non-recombinant clones (blue color). This coloris based on inactivation of the marker beta-galactosidase, preventingcleavage of X-gal (5-bromo-4-chloro-3-indolyl β-galactoside). The cellcolonies harbouring a non-recombinant vector produce a functionalbeta-galactosidase and, by hydrolysing the X-gal substrate, produce ablue coloration. In general, the insertion of a DNA fragment into theβ-galactosidase gene prevents cleavage of the X-gal. For this reason,the cells harbouring a recombinant vector have a white color.

Moreover, this complex procedure requires the use of the substrate X-galwhich is a product which is very expensive, unstable and awkward to use.

On the other hand, various cloning vectors permitting direct selection(positive selection) of recombinant strains have been described in thescientific literature.

Pierce et al (Proc. Natl. Acad. Sci., vol 89. No. 6, 1992, pp.2056-2060) describe a vector which comprises the lethal gene sacB fromBacillus amylolique-faciens, integrated into a plasmid derived from thebacteriophage P1 and under the control of a specific E. coli promoter.

The promoter of this vector includes a region having several specificcloning sites (cleavage site for a restriction enzyme).

Since the gene sacB encodes levan sucrase, which catalyses thehydrolysis of sucrose into products which are toxic for E. coli, directselection of the mutants which incorporate a recombinant plasmid iseffected on a culture medium containing sucrose. Since the levan sucraseis toxic, even in the absence of sucrose, it is essential, consequently,to repress its synthesis if one wishes to obtain a large number ofplasmid copies in the bacterial cytoplasm.

However, it is difficult, if not impossible, to repress the cytotoxicgene completely, particularly if a large number of copies of the vectorare required.

Therefore, the impossibility of repressing the cytotoxic gene leads, inphases of producing the plasmid, to the death of the cell and, as aconsequence, to selective pressure towards mutated strains(characterised by an inactive lethal gene).

In this case, in order to ensure that the enzyme encoded by the sacBgene does not kill the host cell, it is necessary to incorporate a CIrepressor, which regulates the expression of this gene, into the cloningvector.

Furthermore, since sucrose is often incorporated into bacterial culturemedia, it will be essential to prepare media which are totally free ofsucrose in order to carry out these manipulations.

Henrich et al (Gene, vol 42, No. 3, 1986, pp. 345-349) describe a vectorwhich includes the E gene from the bacteriophage φ174, the said E genebeing incorporated into the plasmid pUH84 under the control of the Lacpromoter.

In this case, the E gene includes six unique restriction sites (locatedover the whole of the E gene sequence) and encodes gpE, which causeslysis of the E. coli cell. In this case, positive selection is effectedwhen a foreign recombinant gene has been inserted into one of therestriction sites.

However, this insertion of a foreign gene into a restriction sitelocated in the sequence of the E gene, encoding gpE, makes it moredifficult to sequence the foreign gene and/or amplify it by PCR since,in this case, portions of useless sequences belonging to the E geneencoding gpE are also sequenced, amplified and characterised.

Kuhn et al (Gene, vol 42, No. 3, 1986, pp. 253-263) describe a vectorwhich includes a large gene encoding a restriction enzyme which kills bycleaving the genome of the bacterium, the said gene being incorporatedinto the plasmid pKG2 under the control of the LacUV5 promoter.

The cloning vectors of the state of the art suffer from the disadvantageof having to be maintained in a host strain which includes the LacI^(q)repressor in episomal form, or the CI repressor, in order to inactivatethe promoter and prevent expression of the killer gene, leading to thedeath of the host strain.

In addition, if it is desired to use this strain to produce a largenumber of copies of the cloning vectors, the repressor will not beadequate for preventing either a selective pressure which modifies thecytotoxic activity of the vector or a “genetic leakage”, that is to sayexpression of certain copies of the vector and death of the host strain.

Consequently, none of the documents of the state of the art describes acloning vector which can incorporate large nucleotide fragments, whichis easy to manipulate and which can be produced by a micro-organism onan industrial scale; that is to say, which can be produced in a largenumber of copies by a micro-organism without bringing about the death ofthe latter.

OBJECTS OF THE INVENTION

The present invention aims to supply a novel cloning and/or sequencingvector, and also its host strain, which are simple and relativelyinexpensive to construct and produce, and which enable recombinantclones to be selected directly, without suffering from the disadvantagesof the above-mentioned state of the art.

A particular object of the present invention is to obtain a vector whichpermits specific and certain selection of the recombinant clones.

Another object of the present invention is directed towards obtaining avector which permits the sequencing, amplification and/orcharacterisation, using the same primer, of any foreign DNA fragment(whatever its size) in the recombinant clones.

An additional object of the present invention is directed towardsobtaining a vector which also permits simple extraction of this foreignDNA fragment from the recombinant clone.

A final object of the present invention is directed towards obtaining ahost strain for the said vector which allows a large number of copies ofthe said vector to be produced without bringing about selective pressurewhich modifies the cytotoxic activity of the said vector or causing thedeath of the host strain.

CHARACTERISTIC ELEMENTS OF THE INVENTION

The invention relates to a new cloning vector comprising at least onepromoter nucleotide sequence and at least one nucleotide sequencecomprising several unique cloning sites artificially created, localizedat the level of a nucleotide sequence for a gene encoding a cytotoxicmolecule.

The invention relates to a novel cloning and/or sequencing vector whichincludes, incorporated into an autonomously replicating vector, at leastone promoter nucleotide sequence and at least one nucleotide sequenceencoding a fusion protein which is active as a poison, the saidnucleotide sequence being obtained by fusing a coding nucleotidesequence which includes several unique cloning sites and a nucleotidesequence which encodes a protein poison.

Preferably, the autonomously replicating vector is a recombinant virusor a recombinant plasmid such as a pUC plasmid.

The promoter nucleotide sequence can comprise any promoter, whichpermits expression of the nucleotide sequence encoding a fusion proteinwhich is active as a poison.

Preferably, this promoter nucleotide sequence consists of the Lac operonpromoter.

According to one preferred embodiment of the invention, the uniquecloning sites (MCS) of the nucleotide sequence which is fused to thenucleotide sequence which encodes the protein poison are absent from theremainder of the nucleotide sequence of the vector according to theinvention.

Advantageously, the nucleotide sequence of the gene which encodes theprotein poison comprises all or part of the nucleotide sequence of thewild-type gene which encodes the protein CcdB or the protein Kid.

Preferably, the nucleotide sequence of the gene which encodes theprotein poison lacks the cleavage site for the restriction enzyme SmaI.

Another aspect of the invention relates to a procaryote cell which istransformed with the cloning vector according to the invention.

The invention also relates to a procaryote host cell for the vectoraccording to the invention which possesses a chromosomal I^(q) and anelevated transformation efficiency, and which possesses a mutationconferring resistance to the poison activity of the fusion protein,and/or which possesses a gene encoding a protein which is an antipoisonto the fusion protein.

Preferably, the procaryote host cell for the vector according to theinvention possesses a mutation in the gene encoding subunit A, or in thegene encoding subunit B, of the gyrase, and conferring resistance to thefusion protein, and/or a gene which encodes the protein CcdA which is anantipoison to the fusion protein comprising CcdB and/or encodes theprotein K is which is an antipoison of the fusion protein comprisingKid.

Preferentially, the procaryote cell is an Escherichia coli cell whichpossesses a mutation which is responsible for replacing arginine 462with a cysteine in the amino acid sequence of the GyrA polypeptide ofthe gyrase, thereby conferring resistance to the fusion protein.

Preferably, this procaryote host cell also possesses the LacI^(q)mutation.

The present invention also relates to fragments of the vector accordingto the invention, in particular primers for sequencing and/or amplifying(for example by PCR) the foreign nucleotide fragments inserted into thevector according to the invention.

Preferably, these primers consist of sequences of from 10 to 30nucleotides which hybridise to nucleotide sequences which are situatedon either side of the nucleotide sequence of the vector according to theinvention which contains several unique cloning sites.

A final aspect of the invention relates to the use of the vectoraccording to the invention for selecting and sequencing recombinantclones.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 is a diagrammatic representation of a cloning vector according tothe present invention.

DESCRIPTION OF A PREFERRED EMBODIMENT OF THE INVENTION

According to the invention, the cloning and/or sequencing vector 1includes, incorporated into an autonomously replicating vector 2, atleast one promoter nucleotide sequence 3 and at least one nucleotidesequence 4 which encodes a fusion protein which is active as a poison,the said nucleotide sequence 4 being obtained by fusing a codingnucleotide sequence 5 (or polylinker) which encompasses several(multiple) unique cloning sites (MCS), and a nucleotide sequence (6)which encodes a protein poison.

An autonomously replicating vector 2 is understood to mean anynucleotide construct, such as a virus or a plasmid (preferably arecombinant plasmid of the PUC series), which is capable of beingintroduced into a micro-organism, of recombining therein and/or ofreplicating therein.

FIG. 1 shows a diagrammatic representation of a cloning vector accordingto the present invention, which vector is constructed from a plasmid ofthe pUC series (pUC18 and pUC19), which is described by Norrander et al(Construction of improved M13 vectors usingoligo-deoxinucleotide-directed mutagenesis, Gene, 26, pp. 101-106(1983)) and by Yanisch-Perron et al (Improved M13 phage cloning vectorsand host strains-nucleotide sequences of the M13 mp18 and pUC19 vectors,Gene, 33, pp. 103-119 (1985)).

A coding nucleotide sequence 5 encompassing several (multiple) uniquecloning sites (MCS) is understood to mean a short coding sequence (orpolylinker) which comprises several cleavage sites for restrictionenzymes.

The advantage of having a polylinker in the vector according to theinvention is that different cloning sites are located on a single shortsequence, thereby permitting:

-   -   rapid sequencing and amplification, using the same primers, of        any DNA fragment which is inserted into this vector,    -   rapid extraction of the cloned fragment, facilitated by the        proximity of the restriction sites. Thus, in contrast to the        state of the art, this proximity avoids sequencing, amplifying        and characterising useless fragments from other sequences of the        vector according to the invention.

Nucleotide sequence 6 encoding a protein poison is understood to meanany (wild-type) nucleotide structure encoding a protein which displaysan activity which is naturally poisonous and specific for one or morevital functions of a host cell.

A protein poison is also characterised by the existence of an antidoteor antipoison, such as the proteins CcdB and CcdA, the protein Kid andits antagonist K is, the protein PemK and its antagonist PemI, theprotein Doc and its antagonist Phd, the protein HoK and its antagonistSok, and other poison molecules which are, or are not, of plasmidorigin.

In this case, the nucleotide sequence 6 encoding a protein poisonconsists of the wild-type gene CcdB, which encodes the protein CcdB(control of cell death), obtained from the ccd locus of the F plasmid(SEQ ID NO: 1 and SEQ ID NO:4).

The ccd locus of the F plasmid comprises the two wild-type genes ccdAand ccdB, also termed H and G, or letA and letD, which respectivelyencode proteins of 72 and 101 amino acids (Bex et al, Mini-F encodedproteins; identification of a new 10.5 kilodalton species. EMBO J.2,1853-1861 (1983); Miki et al, Control of cell division by sex factor Fin Escherichia coli. I. The 42.84-43.6 F segment couples cell divisionof the host bacteria with replication of plasmid DNA, J. Mol. Bio., 174,605-625, (1984)).

In Escherichia coli, the CcdB protein of the F plasmid is a cytotoxinwhose lethal activity is counteracted by the protein CcdA (Karoui et al,Ham22, a mini-F mutation which is lethal to host cell and promotesrecA-dependent induction of lambdoid prophage. EMBO J.2, 1863-1868(1983); Ogura and Hiraga Mini-F plasmid gene that couple host celldivision to plasmid proliferation, Proc. Natl. Acad. Sci. USA, 80,4784-4788 (1983); Miki et al, Control of cell division by sex factor Fin Escherichia coli. Identification of genes for inhibitor protein andtrigger protein on the 42.84-43.6F segment, J. Mol. Biol. 174, 627-646(1984b)).

The molecular mechanism by which protein CcdB exerts its lethal activityhas been elucidated; protein CcdB is poisonous to DNA topoisomerase II.

The type II DNA topoisomerases are essential and ubiquitous enzymeswhich alter the topology of the DNA by transiently introducing adouble-stranded break into the DNA. During the stage ofbreak-religation, topoisomerase II forms an intermediate complex withits DNA substrate in which the enzyme is attached covalently to the 5′end of the cleaved DNA, This transitory intermediate, in whichtopoisomerase II is linked covalently to the DNA, has been termed the“cleavable complex” (Wang, DNA topoisomerases. Annu. Rev. Biochem. 54,665-97, 1985; Maxwell & Gellert, Mechanistic aspects of DNAtopoisomerases. Advan. Protein Chem. 38, 69-107, 1986; Liu, DNAtopoisomerase poisons as antitumor drugs, Annu. Rev. Biochem. 58.351-375, 1989).

Both in eucaryotes and in procaryotes, the cleavable topoisomeraseII-DNA complex is the target of powerful therapeutic agents, includingthe antibiotics of the “quinolone” family, which act on the gyrase(bacterial topoisomerase II), and anticancer agents (acridines andepipodophyllotoxins), which act on the mammalian topoisomerase II. Thetherapeutic efficacy of the topoisomerase poisons is correlated withtheir ability to stabilise the cleavable complex.

DNA topoisomerase II is an essential enzyme in all living entities andis very conserved in the evolution of the species. The CcdB protein thusdisplays an activity which is potentially cytotoxic for a wide varietyof procaryote species.

The small size of the wild-type ccdB gene allows it to be inserted intoplasmids without increasing their size excessively and consequentlyallows large fragments of foreign DNA to be included therein,Furthermore, given its small size, the wild-type ccdB gene of the Fplasmid contains very few restriction sites; it is, therefore, simplerto preserve the uniqueness of the multiple cloning sites (MCS) which areadded to it.

Unexpectedly, the inventors observed that the in-phase fusion of thenucleotide sequence 6, encoding protein CcdB, with the coding nucleotidesequence (polylinker 5), comprising several (multiple) unique cloningsites (MCS) gave a nucleotide sequence 4 which encodes a fusion proteinwhich is active as a poison and which makes it possible, as aconsequence, to produce vectors for the direct selection of recombinantplasmids (killer selection).

The plasmids which have been obtained allow doubly digested restrictionfragments to be cloned in both orientations with respect to the lacpromoter. Insertion of a restriction fragment into one of the uniquecloning sites interrupts the genetic information of the gene fusion,leading to the synthesis of a gene fusion product which is notfunctional. Insertional inactivation of the gene fusion ought always totake place when a termination codon is introduced or when a change ismade in the reading frame.

The cells which harbour an intact cloning vector of this nature producea poisonous fusion protein which is functional, and die.

Insertion of a foreign DNA fragment into one of the unique cloning sitesof the gene fusion interferes with production of the poison.

The cells which harbour a recombinant vector will be viable while cellswhich harbour an intact vector will not be viable. This killerselection, by simple culture on a solid medium, makes it possible toeliminate cells which harbour a non-recombinant vector (non-viableclones) and to select recombinant clones (viable clones).

Example I Construction of the Plasmid pKIL19

The ccdB gene was amplified by PCR using, as DNA template, the plasmidpULB2208 (Bernard and Couturier, The 41 carboxy-terminal residues of theminiF plasmid CcdA protein are sufficient to antagonise the killeractivity of the CcdB protein, Mol. Gen. Genet. 226, 297-304 (1991) aswell as synthetic oligonucleotides.

The synthetic oligonucleotide sequences were selected in such a way asto create an EcoRI restriction site on either side of the wild-type ccdBgene in order to be able to reclone this gene in frame with the codonsof the MC S19 multiple cloning site and to eliminate the initiationcodon of the native ccdB gene. The DNA resulting from the PCR reactionwas digested with the enzyme EcoRI and cloned into the EcoRI site of theplasmid pUC19. The resulting plasmid, in which the EcoRI fragment wasintegrated in the orientation which permitted the ccdB gene, providedwith the additional codons corresponding to the MCS19 multiple cloningsites, to be read from the Lac promoter, was termed pKIL2. Plasmid pKIL2is lethal for a wild-type bacterium (Ccdb^(S) sensitive).

pKIL2 also possesses two SmaI sites, one in the multiple cloning sitesand the other in the central region of the ccdB gene. The latter waseliminated by site-directed mutagenesis. The resulting plasmid pKIL3,having a unique SmaI site, still has two EcoRI sites. The EcoRI sitedownstream of the ccdB gene was eliminated by filling in its cohesiveends.

The resulting plasmid, pKIL19 (SEQ ID NO:2 and SEQ ID NO:5), thuspossesses a unique EcoRI restriction site within sequence 5, whichencompasses the multiple cloning site.

Example II Construction of the Plasmid pKIL18

The ccdB gene was amplified by PCR using, as DNA template, plasmidpKIL19 as well as synthetic oligonucleotides. The sequences of thesynthetic oligonucleotides were selected in such a way as to create aHindIII site on either side of the ccdB gene in order to be able toreclone this gene in frame with the codons of the MCS18 multiple cloningsites. The DNA resulting from the PCR reaction was digested by theenzyme HindIII and cloned into the HindIII site of the plasmid pUC18.The resulting plasmid, in which the HindIII fragment was integrated inthe orientation which permitted the ccdB gene, provided with theadditional codons corresponding to the MC S18 multiple cloning sites, tobe read from the Lac promoter, was termed pKIL4. Plasmid pKIL4 is lethalfor a Ccdb^(S)-sensitive bacterium.

The HindIII site downstream of the ccdB gene was eliminated by fillingin its cohesive ends. The resultant plasmid, pKIL18 ((SEQ ID NO:4 andSEQ ID NO:6), possesses a unique HindIII restriction site as well as aunique SmaI site (since constructed from pKIL 19).

Example III Construction of the Plasmid pKID18

ParD is a killer stability system of R1 plasmid located in the proximityof the basic replicon. It is a small operon containing two genes, Kidand K is, coding for a killer component and its antagonist respectively(Bravo et al., Mol. Gen. Genet., Vol. 215, pp. 146-151 (1988)). Thissystem is perfectly conserved and functional in another incFII plasmid,R100 (pem system: Tsuchimoto et al., J. of Bacteriol., Vol. 170, pp.1461-1466 (1988)), PemA (identical to Kis) and PemB (identical to Kid).

The vectors pKID18 and pKID19 contain the Kid gene fused to differentpolylinkers (MCS18 and MSC19 for pKID18 and pKID19 respectively). TheKid sequence was amplified by PCR from the plasmid R1 drd19 using theprimers kid1—gaggaattcattgggaaagaggggaaatctg—(SEQ ID NO:7) andkid2—gaggaattctcaagtcagaatagtggaca—(SEQ ID NO: 8). The generated insertwas cloned into the EcoRI site of pUC19 (Yanish-Perron et al. (1985)).This insertion generates a fusion gene between the MCS19 and Kid. Thevector pKID18 was obtained as follows: the Kid sequence was amplified byPCR from the plasmid R1 drd19 using the primerskid3—gagaagcttattggaaagaggggaaatctg—(SEQ ID NO:9) andkid4—gagaagctttcaagtcagaatagtggaca—(SEQ ID NO:10). The generated insertwas cloned into the HindIII site of pUC18 (Yanish-Perron et al. (1985)).This insertion generates a fusion gene between the MCS18 and Kid.

In induce conditions (induction of the pLac) that control the fuse Kidtranscription of this construct for the E. coli strain (Top-10 FInvitrogen), this vector which contains the Kid gene fused to differentpolylinkers has retained the poison activity of the original Kidprotein.

The regulation and the expression of this vector in a specific cellwhich is not killed by the poison activity of the fusion protein can beobtained by a control of the promoter activity of said vector or can beobtained by the production of said vector in a cell expressing the K isprotein which is the antidote of the Kid protein.

Example IV Construction of the Strains Ccdb^(r) and Ccdb^(S)

In order to be able to maintain plasmids pKIL18 and pKIL19 within abacterium, the latter has to be resistant to the lethal effect of thefusion protein which is active as a poison, Unexpectedly, thechromosomal mutation gyrA462 confers on the strains total resistance tothe poisonous effect of the fusion protein.

Moreover, since plasmids pKIL 18 and pKIL 19 derive directly fromplasmids pUC18 and pUC19 and express the ccdB genes from the Lacpromoter, it is preferable to maintain these plasmids in a LacI^(q)strain. Thus, while, in our case, continuous overexpression of thesegenes does not exert a selection pressure in favour of certainmutations, the LacI^(q) strain allows expression from the Lac promoterto be reduced and conserves the bacterial machinery, therebyguaranteeing a rapid generation time (increased production of the vectorby the strain).

The strain D1210 (Sadler et al Gene 8, pp. 279-300 (1980)), derived fromthe strain HB101 LacI^(q), LacY⁺ (Maniatis et al Molecular CloningLaboratories Man. Cold Spring Harbour Laboratory N.Y.), andcharacterised by a chromosomal I^(q) and increased transformationefficiency, was transformed with the plasmid pCOS2.1. This plasmid,which confers resistance to kanamycin, carries the recA gene fromErwinia chrysanthemi 3665 and allows recombination in E. coli. A lysateof P1 phage was prepared on a Ccdb^(R) gyrA462, zei298::Tn10 strain andused to infect the strain D1210/pCOS2.1. The transductants which wereresistant to tetracycline were selected and tested for their resistanceor sensitivity to the CcdB protein. One of the Ccdb^(R) transductantswas then cured of plasmid pCOS2.1 and termed KIB22.

Strain KI322 constitutes an ideal host strain for plasmids pKIL18 andpKIL19 while strain D1210 constitutes the ideal host for selectingrecombinant plasmids.

Thus, strain KIB22 advantageously possesses an elevated efficiency ofDNA extraction (comparable to the yield of the pUC plasmids) and,unexpectedly, resistance to the fusion protein which is encoded bypKIL18 and pKIL19.

Consequently, it is possible to use this micro-organism to produce thecloning vector according to the invention on an industrial scale innumerous copies without causing the death of the said micro-organism.

The selection is carried out simply by spreading the bacteria on amedium containing IPTG (Isopropyl-β-D-thiogalactopyranoside) as well asampicillin.

Strain KIB22 was deposited with the Laboratorium voorMicrobiologie-Bacterienverzameling (LMG) [MicrobiologicalLaboratory—Bacterial Collection] of the Belgian Coordinated Collectionsof Microorganisms (BCCM) under No. LMG P-12601.

The cloning vector pKIL19 was deposited with the Laboratorium voorMoleculaire Biologie-Plasmiden Collectie (LMBP) [Molecular BiologicalLaboratory—Plasmid Collection] of the Belgian Coordinated Collections ofMicroorganisms (BCCM) under the No. LMBP 2781.

These depositions were made in accordance with the provisions of theBudapest Treaty regarding the International Recognition of theDeposition of Microorganisms.

1. A method of cloning a DNA fragment in a prokaryotic cell, said methodcomprising: providing a cloning vector comprising at least one promoternucleotide sequence and at least one nucleotide sequence comprising atleast one cloning site localized at the level of a nucleotide sequencefor a gene encoding CcdB, said CcdB being cytotoxic; inserting said DNAfragment into said at least one cloning site in said cloning vector suchthat said DNA fragment interferes with the cytotoxic activity of saidCcdB; transferring the cloning vector with the DNA fragment into theprokaryotic cell; and growing the prokaryotic cell to produce multiplecopies of the DNA fragment.
 2. The method of claim 1, wherein said atleast one nucleotide sequence comprises a plurality of cloning sites. 3.The method of claim 1, wherein said at least one cloning site is aunique cloning site.
 4. The method of claim 1, wherein said at least onecloning site is an artificially created cloning site.
 5. The method ofclaim 1, wherein said at least one promoter nucleotide sequencecomprises a repressible promoter.
 6. The method of claim 5, wherein saidrepressible promoter is a Lac operon promoter.
 7. The method of claim 1,wherein said cloning vector is a recombinant virus or recombinantplasmid.
 8. The method of claim 7, wherein said recombinant plasmidcomprises a pUC origin of replication.
 9. The method of claim 8, whereinsaid pUC origin of replication is the origin of replication from pUC18or pUC19.